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The Pursuit family of columns
Pursuit columns are a family of exceptionally high ligand density
bonded phases on ultra high purity Type B silica that deliver:
- Extended pH range
The Pursuit XRs and 200A based Pursuit share similar family
attributes. However, they can be differentiated by features and benefits
as described below. The main decision point comes when the customer
decides if separation speed is needed as in the case of LC/MS or
resolution is the key property such as when using HPLC with UV
detection. The two phases compliment each other by spanning the range of
For speed, Pursuit columns are positioned for LC/MS and other
high-throughput applications. Built on the larger 200A pore silica, high
ligand density delivers up to 40% faster separations without sacrificing
resolution. This is accomplished by optimizing mass transfer with the
If you customer needs load and resolution, Pursuit XRs columns are
positioned for performance in AR&D, QC and preparative applications.
Combining the high ligand density with a smaller 100A high surface area
silica gives maximum loadability and method robustness/reliability. his
is due to an extremely high carbon load (~25% for C18) on the surface.
Pursuit XRs columns
The Pursuit XRs line of HPLC columns is the newest addition to the
Pursuit family of HPLC columns. The XRs combines the high phase density
bonding found in all Pursuit columns with a high surface area 100A
silica. Pursuit XRs delivers better resolution and reliability for the
most demanding applications, from research and development (R&D) to
production and quality control (QC), compared to any column in its
class. Method developers will appreciate the faster development and
validation of new methods, enabled through first-time resolution of
complex mixtures and outstanding peak symmetry of polar compounds.
Quality control laboratories will welcome the outstanding repeatability
and column performance as standout benefits, while all users will value
the peace of mind that comes from owning state-of-the-art performance.
These customers can be found in many market segments including but not
limit to pharmaceutical, environmental and food & beverage.
With Pursuit XRs your customers will see:
- High resolution
- Reproducible results
- Outstanding lifetime
The 3 bonded phases of XRs each deliver a targeted set of benefits.
Pursuit XRs C18, optimized for maximum separation power, is the
preferred choice for resolving complex mixtures. With a 25% carbon load
and exhaustively endcapped surface, XRs C18 is the ideal 'all round'
Key performance benefits:
- Maximum resolution
- Excellent peak shape
- Optimal retention
Pursuit XRs C8, provides enhanced selectivity for low hydrophobicity
compounds and positional isomers, making it the column of choice for
these challenging analytes. For routine isocratic applications, the
shorter C8 ligands also generally provide faster run times than C18.
Key performance benefits:
- Improved shape selectivity
- More symmetrical peaks for polar analytes
- Faster elution
Pursuit XRs Diphenyl, delivers superior resolution of aromatic
compounds, eclipsing the performance of other phenyl or hydrophobic
columns. The highly selective pi-pi retention mechanism, Diphenyl
exploits analyte differences based on their pi-electron structure.
Conventional reverse phases, such as C18 and phenyl hexyl, rely heavily
on hydrophobic interactions and simply lack the selectivity power of XRs
Diphenyl for aromatic compounds.
Key performance benefits:
- Unique pi-pi selectivity
- Enhanced resolution
- Improved peak symmetry
The Pursuit XRs product line has been developed to specifically
target the needs of traditional HPLC customers. These traditional HPLC
operators comprise ~70% of HPLC column consumption as compared to ~15%
by LC/MS users. They can be found in AR&D and QC type groups across
industries ranging from pharmaceutical to environmental to food &
beverage to agriculture and beyond. Their 'point of pain' is based in
the need for baseline resolution from a stable, reliable column. This is
different than an LC/MS operator's needs since the MS can often provide
additional 'resolution' of co-eluting peaks delivering unequivocal
results. Pursuit XRs' high carbon load bonding and large surface area
combine to deliver the resolution and stability these customers need.
By being attentive and addressing your sales discussions towards to
the needs of this large market segment, you will find great success.
For the majority of reversed phase analysis the new Pursuit XRs HPLC
column will increase laboratory productivity. Analytical methods will
benefit from increase in stability, reliability, and rapid method
development compared to the existing column solutions.
Itís important to ask the customer the right questions to correctly
understand their needs and
enable you to position the correct solution. The following questions
will help you to present the
sales arguments that will have maximum impact.
- What types of stationary HPLC columns are you currently using for
analysis? (brand, phase, particle size)
- What part of the methods would you like to see improved?
- What are the most important considerations when you 'update',
develop and validate
methods? (Speed, accuracy, selectivity, sensitivity, reproducibility,
- Would it be an advantage for you if you could perform most
applications on one type of
- Do you have the possibility to select another brand of column within
- Is it beneficial for you to purchase your HPLC columns from one
- Do you practice LC/MS applications?
In order to achieve adequate analytical separations many laboratories
use 4-5 different
types of reverse phase column. This requires the set-up and maintenance
methods making routine operation difficult and expensive.
- Do you think that it would be a good idea that your laboratory would
standardized, for reversed
phase type of analyses, on one type of column?
Pursuit XRs has a extremely high surface area, which result in highest
maximum reproducibility and ultimate method robustness, which makes it
wide range of analytes.
This means that new methods become easier to set-up. Plus many types of
columns, used in existing methods, can be replaced by the new Pursuit
which offers you easier method development and often analytical results
will benefit from
improved accuracy. For pharmaceutical applications this means that the
and itís metabolites can be analyzed in the same run!
Validated analysis can only be achieved by using validated analytical HPLC columns.
- Do you think it is necessary for your work to use highly reproducible
and quality assured HPLC
Every Pursuit XRs column is validated and we guarantee that it will
perform to tight
specifications. We can guarantee this because every step in the
manufacture and testing
of the Pursuit XRs columns is critically controlled. All sources and
processes are monitored and verifiable. Each individual column is QCíd
set to the highest
standards in the industry and is supplied with a certificate of
analysis. This ensures that
the column will can be reliably perform, column after column, batch
after batch, and in
Which means that you improve productivity and gain major cost savings.
It is often problematic and time consuming to change a method from one
another method. Method development is best done based on existing
- Do you think it is an advantage to be able to use this type of support
in the eventual adaptation of
your methods with Pursuit XRs columns?
The Pursuit XRs columns are used in a wide range of application fields,
implementation quite easy. Varian can support you with numerous of
are easy accessible through the Scan Web application database.
Technical Helpdesks provides focused support for your specific
Benefit from Varian chromatography expertise and save a lot of time
developing a new method. So, whether you want a faster separation,
or higher sample turn around you will improve your processes in a
fraction of the normal